阳虚体质者血清和尿液的核磁共振代谢组学

摘要

Nuclear magnetic resonance ( N MR)-based metabonomics was applied to study the yareg-deficiency constitution by analyzing metabolic profiling of serum and urine. The purpose of this study is to determine an array of characteristic metabolites in serum and urine samples from yang-deficiency constitution person and then to interpret these metabolites in possible metabolic pathway. Serum and urine samples from 30 yang-defi ciency constitution person with 30 balanced constitution person were collected. Differences in endogenous metabolites were detected on serum and urine samples from the yang-deficiency constitution group and control group using multivariate statistical analysis. Compared with the balanced constitution, the contents of lactate, very low density lipoprotein( VLDL)/low density lipoprotein ( LDL) , /V-cetyl glycoprotein ( Nac ) , fatty acid (FA) , unsaturated fatty acid (UFA) are lower while the contents of glutamine, glucose, phosphatidylcholine (PtdCho) , high density lipoprotein( HDL) are higher in serum of yarag-deficiency constitution, and the content of creatinine is lower while the contents of lactate, DMA, citrate, hippurate are higher in urine of yang-deficiency constitution. The biomarkers supply new ways for the individual differences from the point of view of metabonomics. Fang-deficiency constitution has some metabolism disorder of energy, lipid and glucose, and also has changes of organs functions.%采用基于核磁共振(NMR)的代谢组学方法研究了中医阳虚体质及平和体质(正常对照)的血清和尿液,分析了阳虚体质的特征代谢物.实验收集阳虚体质及平和体质各30人的血清及尿液样品,采用多元统计分析方法研究了阳虚质组和对照组血清和尿液中的内源性代谢差异,结果表明,阳虚质血清中乳酸、极低密度脂蛋白/低密度脂蛋白、N-乙酰糖蛋白、脂肪酸及不饱和脂肪酸的含量降低,谷氨酰胺、葡萄糖、磷脂酰胆碱及高密度脂蛋白的含量增多；尿液中肌酐的含量降低,乳酸、二甲胺、柠檬酸及马尿酸的含量增多.阳虚体质的潜在生物标志物的发现从代谢组学角度为个体差异提供了新的依据.阳虚体质与平和体质存在能量代谢、脂代谢及糖代谢的差异以及相关脏腑功能的改变.