荧光探针Ru( phen)2(dppx)2+测定H1N1禽流感病毒DNA

摘要

The determination of sequence-specific DNA related to the avian influenza H1N1 virus was realized with the molecular 'Light Switch' complex of Ru(phen)2(dppx)2+. There was no or weak fluorescence between the fluorescent probe Ru(phen)2(dppx)2+ and ssDNA probe. However, the luminescent enhancement was observed in the presence of target DNA resulted from the intercalation of the complex into the hybrids between probe DNA and target DNA. Under the optimum conditions, the linear range of the DNA related to the avian influenza H1N1 virus is 9. 3×10-10-7.4×10-8 mol/L, the calibration curve is y = 3.3829x + 8. 3948, R2 =0. 9982, and the detection limit is 5. 3×10-10 mol/L. This method is simple, rapid, sensitive and selective.%利用荧光探针Ru (phen)2( dppx)2+与ssDNA作用时无荧光或产生弱荧光,而与dsDNA作用时荧光增强的机理,将H1N1禽流感病毒ssDNA和与其完全互补的ssDNA′杂交形成dsDNA实现Ru (phen)2 (dppx)2+对H1N1禽流感病毒DNA特定序列(5′-CTACCATGCGAACAATTCAACCGACACTGTT-3′)的定量检测.在优化的实验条件下,测定H1N1禽流感病毒DNA的线性范围为9.3×10-10 ～7.4×10-8 mol/L,线性方程为y=3.3829x+8.3948,R2 =0.9982,检出限为5.3×10-10 mol/L.该方法具有操作简单、检测快速、灵敏度高和选择好等优点.