KQ, a carbazole-based cyanine dye with aldehyde, was synthesized. Dye KQ showed lower fluorescence quantum yield and no obvious fluorescence responses to the polarity of solvents and bio-macromole-cules such as nucleic acids. Dye KQ was very sensitive to the environmental viscosity and gave strong fluorescence enhancement with the increase of the viscosity. Notably, there is a linear relationship between them. The live cell imaging experiments manifested that KQ could penetrate the cell membrane easily, detect the viscosity of the cellular micro-environment and gave bright and explicit staining image of the inner membrane system of live cells.%合成了一种带有醛基的咔唑类菁染料KQ,其自身的荧光量子产率较低,对极性的敏感性小,且荧光信号不受核酸等生物分子的干扰.KQ对环境黏度有很好的荧光响应,相对荧光强度随着环境黏度的增大而增强,并且在1×10-3 ～1.3216 Pa·s黏度范围内,染料的荧光强度与溶液的黏度呈良好的线性关系.活细胞荧光成像实验结果表明,染料KQ具有良好的细胞膜通透性,并可对细胞内不同位置的黏度检测成像.
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