HPLC-MS法分离与表征微囊藻毒素

摘要

蓝藻水华对淡水湖泊的污染近年来逐渐加剧,其释放的主要有害物质为微囊藻毒素.藻毒素异构体的分离与制备研究对供水安全具有重要的意义.从滇池蓝藻中提取了微囊藻毒素的几种常见异构体并采用高效液相色谱手段对各异构体进行了分离.探讨了不同色谱条件对分离结果的影响,其中的5种异构体得到了很好地分离,使用HPLC-MS对3种含量较多的异构体进行了表征.%The pollution of freshwater lakes caused by algal blooms which can release the microcystins (MCs) became serious in recent years.The separation and characterization of MCs isomers are very important to the research of water supply security.MCs isomers were extracted from cyanobacteria of Dianchi Lake and separated by high performance liquid chromatography.Different ratio of extract solvent and chromatographic conditions were discussed.Five MCs isomers were successfully extracted.Three of which were characterized by HPLC- MS.