高效液相色谱-光电二极管阵列法测定虾青素的含量

摘要

A method using high-performance liquid chromatography (HPLC) coupled with photodiode array detector (PDA) was established for determination of astaxanthin. The separation was performed on Purospher STAR RP 18 column (4.6 mm×250 mm,5μm) with the mobile phase of methanol-water (Volume ratio was 95∶5). The detector was photodiode array detector and the variable wavelength detector (VWD) was set at 482 nm. The column temperature was 30℃and the injection volume was 20μL. The calibration curve was linear in the range of 0.2-16μg/mL,and the correlation coefficient was 0.999 9. The relative standard deviation of determination results was 0.42%(n=6). The average recovery was 100.4%, and the detection limit was 0.01μg/mL. This method is characteristic of rapidity,accuracy,high sensitivity and good reproducibility.%建立虾青素含量测定的高效液相色谱-光电二极管阵列法。采用Purospher STAR RP 18(4.6 mm×250 mm，5μm)色谱柱，以甲醇-水(体积比为95∶5)为流动相，流速1.0 mL/min，检测波长为482 nm，柱温为30℃，进样量为20μL。在所选定的液相色谱条件下，虾青素主峰与其它杂质峰分离良好，虾青素在0.2～16μg/mL范围内线性良好，线性相关系数r=0.9999，检出限为0.01μg/mL，测定结果的相对标准偏差为0.42%(n=6)，平均回收率为100.4%。该法分析快速准确、灵敏度高、重现性好。