迷迭香中酚类物质的微波辅助提取工艺研究

摘要

Amicrowave–assisted extraction procedure was optimized for the extraction of total phenols from rosemary(Rosmarinus officinalisL.) leaf in order to achieve the highest efficiency in this study. Effects ofmaterial particle size,ethanolconcentration,temperature,extraction time and solid–liquid ratio on the yield of total phenols from rosemary leaf usingmicrowave–assisted extraction were investigated by single–factor experiment.And then the optimization ofmicrowave–assisted extraction was performed using orthogonal experimental design principle.The optimumconditions were volumeconcentration of 80% ethanol,extraction temperature of 55℃,extraction time of 30min,solid–liquid ratio of 1∶12(g/mL). Under theseconditions,the yield of the total phenols was 4.30%±0.124%.%为探寻迷迭香中酚类物质更有效的提取工艺，以迷迭香干叶为原料，采用微波辅助提取迷迭香中的酚类物质，通过单因素试验研究物料粒径大小、乙醇浓度、温度、提取时间和料液比对迷迭香酚类物质提取率的影响。在单因素试验的基础上，通过正交试验优化迷迭香酚类物质的微波提取工艺，得其最佳工艺为乙醇浓度80%、提取温度55℃、提取时间30 min、料液比1∶12（g/mL），此条件下，提取率可达到4.30%±0.124%。