马铃薯块茎形成相关基因STI-LIKE在拟南芥植株发育中的功能验证

摘要

A series of gene’s expression and silencing were involved in potato tuber formation. The full length of potatoSTI-LIKE gene was cloned by using the RT-PCR(reverse transcription PCR)method from the potato variety “Atlantic”. The expression ofSTI-LIKE gene in all potato organs were analyses by real time PCR analysis. The results showed thatSTI-LIKE gene expressed in all potato organs. The bioinformatics analysis indicated that STI-LIKE protein contains three TPR domains, and the STI-LIKE gene was homologous recombination in higher plants. The expression vector(pBI121)and interference vectors(pHANNIBAL and pART27)were constructed and transferred to Arabidopsis thaliana. The STI-LIKE protein expression level was examined by Western blot with specific antibody. The results indicated that STI-LIKE gene might be associated with the growth and development of seedling ofArabidopsis.%马铃薯块茎的形成涉及一系列基因的表达和关闭。以马铃薯普通栽培品种“大西洋”为试验材料，采用RT-PCR扩增获得马铃薯STI-LIKE基因全长片段。RT-PCR定量分析表明，该基因在马铃薯营养器官中均有表达。生物信息学分析表明STI-LIKE蛋白具有3个TPR结构域，在高等植物中具有高同源性，是一个普遍存在的蛋白质。为验证STI-LIKE蛋白在拟南芥植株发育中功能，分别构建该基因强启动子表达载体（pBI121）和干扰载体（pHANNIBAL和pART27），转化拟南芥获得了两种载体的拟南芥转基因植株，同时制备STI-LIKE蛋白抗体验证转基因植株蛋白表达。研究结果表明STI-LIKE基因可能参与拟南芥株型发育过程。