沙冬青脯氨酸转运体基因的克隆及表达分析

摘要

通过RACE技术从沙冬青中克隆获得了脯氨酸转运体基因AmProT(GenBank登录号为KJ873133).序列分析表明, AmProT基因开放阅读框为1 329 bp,编码442个氨基酸,预测蛋白质分子量为48.07 kD,等电点为9.32,含有11个跨膜区域,具有典型的脯氨酸转运蛋白的特征.进化分析显示,AmProT与大豆ProT的相似性达到86%.荧光定量PCR分析表明,AmProT在地上部的表达量要显著高于地下部,在受到干旱、高盐、脱落酸胁迫后表达量呈上调趋势,推测AmProT可能在沙冬青响应干旱、盐等非生物胁迫过程中发挥作用.%The cDNA of proline transporter gene inAmmopiptanthus mongolicus(GenBank accession No. KJ873133)was amplified by RACE technology. Bioinformatics analysis showed that the length of AmProT ORF was 1 329 bp, the gene encoded a putative polypeptide of 442 amino acids with a calculated molecular mass of 48.0733 kD and a theoretical pI of 9.32. It had 11 transmembrance domains, which is the typical characteristics of proline translocator. Phylogenic analysis indicated that AmProT was 86% similar to proline transporter gene of Glycine max. Real-time PCR analysis revealed that AmProT expressed in all tissues examined with more higher level in the above-ground part than the underground part; expression profiles under different stress treatments such as drought, salt and ABA were compared, and the results revealed that transcriptional level of AmProT was up-regulated. It is speculated that AmProT plays an important role in response to abiotic stresses such as drought and salt.