香蕉水通道蛋白 SIP2-1基因的克隆和表达分析

摘要

In the present study,we isolated an aquaporin gene from banana,and designated as MaSIP2-1. MaSIP2-1’s complete ORF was 717 bp,and it encoded 239 amino acids. Alignment of amino acid sequences and phylogenetic analysis indicated that the protein encoded by MaSIP2-1 was in high similarity with AQP-encoding protein in the other known plants ;and highly homologous with amino acid sequences in Musa acuminate,Elaeis guineensis,Jatropha curcas,and Camellia sinensis by 98%,74%,65%,and 63%,respectively. Organ-specific analysis showed that MaSIP2-1 constitutively expressed in roots,stems,leaves,flowers and fruits,and the highest in stems. Stress analysis demonstrated that MaSIP2-1 responded to the stress such as the drought,salt and waterlogging.%从香蕉中克隆了一个水通道蛋白（AQP）基因 MaSIP2-1。序列分析表明，该基因存在一个完整的开放阅读框（ORF）717 bp，编码239个氨基酸。多序列比对和进化树分析表明，MaSIP2-1所编码的蛋白与其他植物中 AQP 编码的蛋白具有较高的一致性。其中与马来西亚野生香蕉、油棕、麻风树、野茶树的 AQP 编码的氨基酸序列的同源性较高，分别为98%、74%、65%和63%。器官特异性分析表明，MaSIP2-1在香蕉的根、茎、叶片、花和果实中均有所表达，其中在茎中表达量较高。通过对其在干旱、高盐、低温、涝害胁迫下的表达结果分析显示，该基因响应干旱、高盐、涝害3种胁迫。