马占相思树皮中原花色素提取工艺及其生物活性的研究

摘要

The optimum extraction conditions of three extraction methods of proanthocyanidins from Acacia mangium Bark were investigated by using orthogonal experiment. The extracted results were compared. Then, the vitro antioxidant and anti-tumor activities of different solvent extracts from ultrasonic extracts were studied. The optimum crafts for traditional solvent extraction method were 60% ethanol,70℃,70 min and ratio of material-liquid 1∶18 (g∶mL,the same below),and the obtained yield of proanthocyanidin was 7.42%.The optimum crafts for microwave-assisted extraction were 60% ethanol,8 min for microware time, ratio of material-liquid 1∶18, microwave power 200 W, with the yield of proanthocyanidin 7. 05%. And the optimum crafts for ultrasonic-assisted extraction method were 70 % ethanol, ultrasonic time 50 min, ratio of material-liquid 1∶16, extraction temperature 50℃,and the yield of proanthocyanidin was 8.57%.Result of ultrasonic-assisted extraction was the best,due to the low extract temperature and high yield.The results of antioxidant test showed that the ethyl acetate extracts from ultrasonic-assisted extracts exhibited the highest antioxidant activity,as well as the inhibitory concentration 50%( IC50 ) of DPPH.radical scavenging was 17. 94 mg/L, and it was lower than that of vitamin C ( Vc ) . Experimental results of antitumor activity of different solvent extracts showed that the ethyl acetate extract possessed highest antitumor activity for human hepatoma cell Bel-7404 with the IC50 of 4.86 mg/L,which was similar to antitumor medicine etoposide.%通过正交试验优化了马占相思树皮中原花色素的3种提取方法的最佳提取工艺条件。结果显示,传统溶剂提取法的最佳工艺为：60％乙醇、70℃、70 min、料液比1∶18( g∶mL,下同),原花色素得率为7.42％；微波辅助提取法的最佳工艺条件为：60％乙醇、微波功率200 W、微波时间8 min、料液比1∶18,得率为7.05％；超声波辅助提取法的最佳工艺为：70％乙醇、超声波温度50℃、超声波时间50 min、料液比为1∶16,得率为8.57％。超声波辅助提取法效果最好,浸提温度低,得率高。进一步对超声波提取物不同溶剂萃取物的体外抗氧化活性和抗肿瘤活性进行了研究。结果表明,乙酸乙酯萃取物对DPPH•清除效果最好,其半数抑制浓度( IC50)为17.94 mg/L ,低于对照样维生素C( Vc)的IC50值；乙酸乙酯萃取物对人肝癌细胞Bel-7404具有明显的抑制效果,IC50值为4.86 mg/L,与抗肿瘤药依托泊苷效果相当。