The experiment was performed on neurons freshly isolated from rat DRG by using whole cell patch clamp techniques. When 60 cells responded to GABA were treated with SKF38393, a selective agonist of dopamine Dl receptor, it was found that except one cell which exhibited a slight potentiation( l0.0%,l/60), the inhibition by SKF38393 of GABA-activated current was predominant (88.33%,53/60), while the remainders were of no effect( l.66%,6/60). In 50 neurons tested , when the neurons were treated with R(-)-NPA prior to the application of GABA for 30sec, It was found that GABA-activated membrane currents were inhibited (76%,38/50) , enhanced (2%,l/50) , no effected markedly (22%,11/50). The effect of SKF38393 and R(-)-NPA was dependent on the concentration of SKF38393 and R(-)-NPA . By intracellular application of 10-4mol/L H7, a potent inhibitor of protein kinase , via micropipette the inhibition of SKF38393 and R(-)-NPA was abolished apparently as compared with the control .%应用全细胞膜片钳技术在大鼠新鲜分离DRG神经元上观察多巴胺D1、D2受体选择性激动剂SKF38393和R(-)-NPA对GABAA受体激活电流的影响。在60个对GABA反应的细胞，预加SKF38393 30s后观察到对GABA-激活电流幅值的影响：多数为抑制(53/60)，少数为增强(1/60)和无明显作用(6/60)。另外对GABA敏感的50个受检细胞, 预加R(-)-NPA 30s后对GABA-激活电流幅值的影响也是多数为抑制(38/50)，少数为增强(1/50)和无明显作用(11/50)。SKF38393及R(-)-NPA对GABA(10-4mol/L)-激活电流的抑制作用为浓度依赖性的。上述抑制可为胞内透析蛋白激酶抑制剂H7所翻转。
展开▼
