葡萄籽原花青素减轻小鼠急性化学性肝损伤

摘要

目的 探讨葡萄籽原花青素对小鼠化学性肝损伤的影响.方法 建立两种小鼠化学性肝损伤的动物模型,给药组用不同剂量葡萄籽原花青素灌胃.采用羟胺法、硫代巴比妥酸法分别测定各组肝脏组织SOD活性、MDA含量,全自动生化分析仪检测血清中ALT、AST含量,HE染色法观察肝脏组织病理变化.结果 成功复制小鼠CCl4和酒精性肝损伤动物模型,SOD活性由对照组(874±41)U/mgprot分别降至两模型组的(265±25)、(225±19)U/mgprot(P＜0.05),葡萄籽原花青素组最高可分别升至(628±36)、(469±16)U/mgprot(P＜0.05).与对照组相比,模型组MDA、ALT、AST含量也明显升高(P＜0.01).与模型组相比,葡萄籽原花青素呈剂量依赖性降低MDA、ALT、AST含量(P＜0.01),同时可明显减轻肝脏组织变性及坏死程度.结论 葡萄籽原花青素可减轻小鼠CCl4肝损伤和酒精性肝损伤.%Objective To investigate the protective effect of grape seed procyanidin on chemical hepatic injury in mice. Methods A chemical hepatic injury mice was developed model with alcohol and carbon tetrachloride respectively ; treating the mice with different concentrations of grape seed procyanidin ( GSP) by intragastric administration as the treatment group; then to detect the serum concentration of alanine aminotransferase ( ALT) and aspartate aminotransferase (AST) ; to analyze the content of superoxide dismutase (SOD) and malondialdehyde (MDA) and to observe the pathological change in the hepatic tissue. Results Two types of acute chemical hepatic injury model were obtained successfully and all indexes in the model groups differed from the control group significantly ( P < 0.01). Compared with the model groups, the content of MDA and serum concentration of ALT and AST in the treatment group decreased dose-dependently (P <0. 05) , but SOD increased in a dose-dependent way. Conclusion GSP exerts protective effect on the alcohol or carbon tetrachloride-induced chemical hepatic injury in the mouse.