内脏高敏感大鼠结肠Cajal间质细胞C-KIT表达增加

摘要

目的 检测内脏高敏感大鼠结肠cajal间质细胞(ICC) C-KIT表达,探讨ICC在内脏高敏感中的作用.方法 取出生后8～21 d的Wistar大鼠,实验组每天直肠注射0.6％的冰醋酸0.3 ～0.5 mL,对照组给予同样剂量的0.9％氯化钠注射液.8周龄时行直肠扩张评估内脏敏感性,记录腹部回缩反射(AWR)为3分时的注水量.取降结肠组织,行C-KIT免疫组化和Western blot检测.结果 与对照组相比,实验组大鼠AWR为3分时的注水量明显减少(P ＜0.05)；C-KIT阳性细胞数在实验组大鼠结肠中(23.37±1.88)较对照组(15.33±1.57)明显增加(P＜0.05)；C-KIT蛋白表达在实验组(134.5±47.5)较对照组(40.6±30.5)明显增强(P＜0.01).结论 ICC增多可能是内脏高敏感的发病机制之一.%Objective To investigate the role of ICC in the pathophysiology of visceral hypersensitivity. Methods 8 to 21 days after birth, the Wistar rats of the experimental group were injected with recta glacial acetic acid 0. 6% 0. 3 -0. 5 mL, the control group were injected the same dose of saline. The visceral sensitivity of 8-week-old rats was evaluated by colorectal distention, and recorded the water injection rate when the score of abdominal withdrawal reflex ( AWR) was 3. The distal descending colon was taken to detect for C-KIT immunohistochemisty and Western blot. Results Compared with the control group, the water injection rate of the experimental group showed a significant decrease when the score of AWR was 3; C-KIT masculine cells counting in colon in the experimental group significantly increased; The expression of C-KIT protein in colon in the experimental group significantly increased. Conclusions The increase of ICC of rat with visceral hypersensitivity may be a possible cause of visceral hypersensitivity.