Objective To investigate the major metabolic changes in low molecular weight serum metabolites of obesity resistant ratsand control rats, understanding the pathogenesis of non-obese type 2 diabetes. Methods A obesity resistant rat model was developed and the biochemical criteria were analyzed. The metabolites in the serum were separated and detected by GC-MS. Multivariate metabonomics analysis was performed after peak alignment. Results Compared with control rats, the obesity resistant rats showed clear metabolism dysfunctions of glucose, lipid and amino acid, pyruvate, succinate, fumarat, citric acid, myristic acid, uric acid decreased. Serum sugar, insuline, low-density lipoprotein and total cholesterol was significantly increased, high density lipoprotein, triglycer-ide decreased significantly. Conclusions After 16 weeks of dietary intervention, the energy metabolism of obesity resistant rats shows significant change. The metabolite profiling study may be helpful for understanding mechanism of non-obese type 2 diabetes.%目的 探讨非肥胖型2型糖尿病的发病机制.方法 建立肥胖抵抗大鼠模型,取静脉血,测生化指标,对血清进行代谢组学分析.结果 大鼠在高糖高脂饮食饲养16周后,与基础饲料组相比,血糖、胰岛素、低密度脂蛋白及总胆固醇显著升高,高密度脂蛋白显著下降,同时糖酵解产物丙酮酸降低,三羧酸循环的中间代谢物柠檬酸、苹果酸、延胡索酸及琥珀酸减少,尿酸增加,脂肪酸、氨基酸代谢异常.结论 高糖高脂饮食干预后,肥胖抵抗大鼠能量代谢显著变化,对探讨饮食导致的非肥胖型2型糖尿病发病机理的研究有重要意义.
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