甲状腺激素自身抗体干扰甲状腺激素的检测

摘要

目的研究甲状腺激素自身抗体干扰甲状腺激素检测的原因及其测定方法,为临床正确判断甲状腺激素水平提供帮助.方法通过自身抗体结合实验、自身抗体稀释度实验、自身抗体抑制曲线实验,自身抗体检定实验和放射性电泳,甲状腺激素自身抗体被测定和鉴定.结果患者血清与125I-T3或125I-T4呈特异性结合,结合率分别为58.77%和49.05%,是正常人或对照的8～13倍,而与125I-TSH的结合是非特异的;得到满意的T3或T4自身抗体稀释度曲线和抑制曲线,病人血清中甲状腺激素自身抗体放射性峰与兔抗人T3或T4抗体出现在电泳同一位置,并被确定为IgG.结论患者体内存在着内源性抗甲状腺激素自身抗体是造成测量值假性升高,干扰激素测定的主要原因.检测甲状腺激素自身抗体有利于正确判断甲状腺激素水平,为临床诊断和治疗甲状腺疾病提供帮助.%Objective To detect and investigate how thyroid hormone autoantibodies interfere in thyroid hormone assays. Methods The binding reaction, dilution testing, calibration curves and the check analysis were performed. Results Thyroid hormone autoantibodies were measured and identified. The combination of the auto-antibodies with 125I-T3 or 125I-T4 were specific, the binding rates were 58.77% and 49.05% respectively, 7-12 times higher than control groups, while with 125I-TSH was non-specific. The radioactive peaks of the autoantibodies and rabbit anti- T3 or T4 antibody appeared at the same position. These antibodies were considered as IgG. Conclusions The presence of anti-thyroid hormone antibodies is the primal cause of falsely high concentrations of serum thyroid hormones. Determination of thyroid hormone autoantibodies is importance in the diagnosis and treatment of thyroid disease.