高效液相色谱法测定土壤和烟叶中精喹禾灵的残留量

摘要

建立了测定土壤和烟叶中精喹禾灵残留量的高效液相色谱分析方法.土壤和烟叶样品中精喹禾灵用丙酮提取,经弗罗里硅土净化后,采用XB-C18柱(150 mmx4.6 mm,5.0μm),以甲醇-水(80:20,v:v)为流动相,流速为0.6 mL/min,柱温为25℃,检测波长为236 nm,采用外标法定量.精喹禾灵的浓度在0.05～5.00 mg/L范围内时,其线性关系良好(R2=0.9974),回收率在85.47％～106.63％,相对标准偏差在0.71％～4.26％.该方法操作简便,灵敏度、准确度和精密度高,能满足农药残留量分析与检测技术的要求.%An analytical method was established to determine the residue of quizafop-p-ethyl in soil and tobacco by high performance liquid chromatography. The residues in soil and tobacco was extracted with acetone,and the extract was cleaned up by a Florisil chromatography column. The separation was per-formed on a C18 column( 150 mm×4.6 mm,5.0 μm)at25℃ by HPLC with an ultraviolet dector(UVD) at 236 nm,and methanol-water(80:20,v:v) as the mobile phase at a flow rate of 0.6 mL/min. The quan-tification was performed by external standard. The calibration curve was linear (R2 =0. 997 4) between the peak area and the concentration when its concentration ranged from 0. 05 mg/L to 5. 00 mg/L. The average recoveries were between 85.47%～106. 63% with its relative standard deviations of 0. 71%～ 4. 26% . This method was characterized by simplicity,sensitivity and accuracy,and could meet the techno-logical acquirement of pesticide residue analysis.