The degradation matching experiments of Yunnan Zhaotong lignite with five kinds of actinomy-ces had been investigated,showing that Streptomyces viridosporous was the most effective degrading actino-myces. Based on single factor experiments,the process conditions of Streptomyces viridosporous degrading Zhaotong lignite were optimized by orthogonal experiments. The results showed that the effect-weight order of the factors to degradation rate were coal particle size > coal pulp concentration>inoculum size>incu-bation time,the best degrading technological conditions were coal particle size of -1003+500 μm,coal pulp concentration of 0. 2000 g/20 mL,inoculum size of 1. 8 mL/20 mL,incubation time of 20 d. Under this technological conditions,the conversion rate reached 42. 56％. Infrared analysis showed that coal had been disappeared or weakened,which further indicated that organic macromolecular in coal had been de-graded by Streptomyces viridosporous into micromolecule containing in the black oil filtrate derived from the degradation. The total ion chromatogram of ethyl acetate extraction product of degradation filtrate dem-onstrated that it contained abundant small molecule organics, which mostly included aliphatic hydrocar-bons,esters,aldehyde,ketone,amine and aromatic compounds,etc.%对五株放线菌和硝酸氧化的云南昭通褐煤进行菌-煤匹配降解实验,筛选出降解优势菌绿孢链霉菌.在单因素实验的基础上,通过正交实验优化了绿孢链霉菌降解硝酸氧化昭通褐煤的工艺条件.结果表明,影响权重为:煤样粒度>煤浆浓度>接种量>培养时间,最佳工艺条件为:煤样粒度-1003+500μm,煤浆浓度0.2000 g/20 mL,接种量1.8 mL/20 mL,培养时间20 d,转化率42.56％.红外分析表明,煤中的有机大分子已被绿孢链霉菌降解成小分子.滤液乙酸乙酯萃取物的GC-MS总离子色谱图证明了降解液中含有丰富的小分子有机物,且大多为脂肪烃、酯、醛、酮、胺及芳香族化合物等.
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