在pH 10.0的磷酸盐缓冲溶液中，以单壁碳纳米管修饰的玻碳电极作为工作电极检测盐酸二甲双胍，在电位0.20 V处表现出一对可逆性较好的氧化还原峰。在此基础上，为了进一步提高检测灵敏度，考察了不同类型的表面活性剂对盐酸二甲双胍电化学行为的影响。结果表明，通过在检测液中添加阳离子表面活性剂十六烷基三甲基溴化铵可以显著提高检测的灵敏度，并在表面活性剂存在下，用方波脉冲伏安法实现了盐酸二甲双胍的电化学测定，其响应电流与盐酸二甲双胍浓度在0.2~5 mmol/L范围内呈现良好的线性关系，与先前无表面活性剂存在下建立检测盐酸二甲双胍的电化学方法相比，检测限降至30μmol/L。并利用该电化学传感器对加标血浆样品进行检测，回收率较好。该方法为简便、迅速测定盐酸二甲双胍提供了一种新思路。%In phosphate buffer solution ( pH 10 . 0 ) , metformin hydrochloride was determined by using electrochemical method with single-wall carbon nanotubes modified glassy carbon electrode as the working electrode,and a pair of well-defined reversible redox peaks located at the potential of 0. 20 V. On this ba-sis,in order to improve the sensitivity,the effect of different type of surfactants was investigated on the electrochemical behavior of metformin hydrochloride. The results showed that cationic surfactant cetyl tri-methyl ammonium bromide ( CTAB) can significantly improve the detection sensitivity,and in the pres-ence of CTAB,square wave pulse voltammetry was applied for electrochemical determination of metformin hydrochloride and the response current has a good linear relationship with the concentration of metformin hydrochloride within the range of 0. 2 ~5 mmol/L. Compared with our previous surfactant-free report about detecting metformin hydrochloride,the detection limit droped to 30 μmol/L. The method can be ap-plied to the determination of Met in spiked plasma samples with good recovery,which provides a new idea for fast,simple determination of metformin hydrochloride by using electrochemistry method.
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