小鼠骨髓染色体G显带最佳制备方法的探究

摘要

[Objective] An improved preparation technology of G-banding chromosome sample was introduced. [Method] Mouse bone marrow cells were used, some key steps such as pancreatic enzyme, staining methods were improved, and the best experimental condition and methods were obtained. [Result] More and better scattered mitosis figures were obtained, and a high practical, simple and easy preparation technology of chromosome of G-banding with clear banding pattern and stable results was established. [Conclusion] The research provides a practical reference for the specification of mice chromosome specimen preparation methods, and improves the experimental teaching effect.%[目的]介绍一种改进的染色体G显带标本制备技术。[方法]采用小鼠骨髓细胞，改进取材方法、玻片的制备、胰酶处理时间以及染色方法等关键步骤，获得了染色体标本制备的最佳处理条件和试验方法。[结果]获得了更多的分散较好的分裂相，建立了一套实用性强、带型清晰、结果稳定、简单易行的染色体G显带制片技术。[结论]结果为规范小鼠染色体标本的制备方法，提高实验教学效果提供了参考。