目的 建立用反相HPLC法测定桦黄中麦角甾醇含量的方法.方法 采用CAPCELL PAK C18色谱柱 (4.6 mm×150 mm,5 μm) ;甲醇为流动相,流速1.0 ml·min-1;柱温40℃,检测波长283 nm.结果 麦角甾醇对照品溶液进样量在0.050 56～1.516 8 μg范围内,与峰面积呈良好线性关系,回归方程为Y=1.49×106X-9 245.2,r＝0.999 9.样品平均回收率为98.17%,RSD为2.83%,样品粉末加甲醇超声处理60 min,制成供试品溶液.结论 该法结果准确、简便可行、分析速度快、分离效果好,适用于桦黄中麦角甾醇的含量测定.%Aim To establish an RP-HPLC method of Ergosterol determination in HuaHuangin. Methods CAPCELL PAKC C18 column (250 mm x4.6 mm,5 μm)was used with a, mobile phase of methanol. The flow rate was 1. 0 ml · min -l and the detection wavelength was set at 283 nm with a column temperature of 40℃ . Results The linear ranges of the determination of Ergosterol was 0. 050 56 ～1. 516 8 μg The regression equation was Y = 1. 49 x 106X _ 9 245 . 2 , r = 0. 999 9. The average recovery of Ergosterol was 98. 17％ and RSD was 2. 83％ . Powder samples in methanol for 60 minutes ultrasound treatment made the test solution. Conclusion The method is accurate , simple ,convenient ,fast and the effect of separation is good , so it can be suitable for Ergosterol determination in HuaHuangin.
展开▼
