目的 探讨整合素β1和β3在胃癌及癌前病变中的表达及其生物学行为的关系,为揭示胃癌发生和发展机制、进一步鉴定胃癌早期诊断的标志物和未来胃癌的药物研发提供理论依据.方法 采用SP免疫组化方法检测整合素β1和β3在45例胃癌及24例非典型增生、20例慢性萎缩性胃炎伴肠上皮化生和25例慢性浅表性胃炎中的表达情况.结果 (1)整合素β1、整合素β3的阳性表达率,胃癌(88.9%、77.8%)明显高于非典型增生(62.5%、54.2%)(P＜0.05);非典型增生明显高于慢性萎缩性胃炎伴肠上皮化生(60.0%、45.0%)(P＜0.05);慢性萎缩性胃炎伴肠上皮化生明显高于慢性浅表性胃炎(32.0%、20.0%)(P＜0.05);(2)胃癌整合素β1、整合素β3的阳性表达率与患者年龄、性别无关(P＞0.05),而与分化程度、浸润深度、淋巴结转移和临床分期有关(P＜0.05);胃癌整合素β1、整合素β3的阳性表达率,低分化组(100.0%、89.8%)明显高于高一中分化组(80.7%、69.2%)(P＜0.05);浸润至浆膜外组(95.8%、91.7%)明显高于浸润至浆膜组(80.9%、61.9%)(P＜0.05);有淋巴结转移组(100.0%、90.9%)明显高于无淋巴结转移组(85.2%、73.5%)(P＜0.05);III～Ⅳ期组(96.0%、88.8%)明显高于I～Ⅱ期组(80.0%、65.0%)(P＜0.05);(3) 整合素β1、整合素β3蛋白的阳性表达强度在胃癌中具一致性(KW=5.734,P＞0.05).结论 整合素β1和β3表达升高与胃癌发生密切相关;两者的表达在胃癌发展中发挥重要的作用.%Aim To investigate the expression of Integrin β1 and Integrin β3 in the process of malignant transformation of gastric cancer,and to explore the relationship among the expression of Integrin β1 and Integrin β3 in gastric carcinoma development and progression in order to provide the theoretic basis for further validating potential molecular targets and gastric cancer diagnosis and screening, as well as the development of gastric cancer drugs. Methods Integrin β1 and integrin β3 were detected by immunohistochemistry two steps methods in 45 cases of gastric carcinomas,24 cases of dysplasia,20 cases of chronic atrophic gastritis with intestinalmetaplasia and 25 cases of chronic superficial gastritis. Relationship among the expression of integrin β1 and integrin β3 and carcinomas differentiation,the depth of invasion, TNM stage and lymph node metastasis was also analyzed. Results The positive ratios of Integrin β1 and Integrin β3 expression in gastric carcinomas (88. 9％ and 77.8％ ) were higher than those in dysplasia(62.5％ and 54.2％ ) (P ＜ 0.05). Occurrence dysplasia was higher than in chronic atrophic gastritis with intestinal metaplasia(60.0％ vs 45.0％ ). Chronic atrophic gastritis with intestinal metaplasia occurred higher than chronic superficial gastritis (32.0％ vs 20.0％ ) (P ＜ 0.05). In gastric cancers the positive intensities of Integrin β1 and Integrin β3 expression were not correlated with age and sex (P ＞ 0.05), but associated with differentiations, depth of tumor invasion,lymph node metastasis,and clinical stages (P ＜ 0.05). Positive expression rates of Integrin β1 and Integrin β3 protein in poorly differentiated gastric cancer( 100. 0％ and 89.8％ )were higher than those in highly and moderately differentiated ones (80.7％ and 69.2％ ) (P ＜ 0.05). Those in gastric cancer with all invasion depth to the outer serosa(95.8％ and 91.7％ )were higher than the ones with an invasion depth to inner serosa(80.9％ and 61.9％ ) (P ＜ 0.05). Those in gastric cancer with lymph node metastasis ( 100. 0％ and 90.9％ ) were higher than the ones without lymph node metastasis (85.2％ and 73. 5％ ) (P ＜ 0.05). Those in gastric cancer of stage Ⅲ ～ Ⅳ (96.0％ and 88. 8％ ) were higher than the ones in gastriccancer of stage I ～ Ⅱ (80.0％ and 65.0％ ) (P ＜ 0.05). The expressions of Integrin β1 and Integrin β3 in gastric cancer had consistency(KW 5.734,P ＞ 0. 05). Conclusion The results suggest that the expression of Integrin β1 and Integrin β3 are correlated with the promotion of gastric carcinomas. They will probably be new indices used for screening and diagnosis of gastric carcinomas.
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