高效液相色谱法测定风湿骨痛丸中双酯型生物碱的限度

摘要

Objective To establish an HPLC method for determing three alkaloids ( aconitine,hypaconitine and mesaconitine ) in Fengshigutong pills. Methods The separation was performed on a Phenomenex C18 column(5 μm,4.6 mm×250 mm) with column temperature of 30℃. The mobile phase consisted of acetonitrile-trahydrofuran-0. 1 mol· L-1 ammonium acetate (per 1 000 mL contains 0. 5 mL) (12. 5=7. 5:80)at the flow rate of 1.0 mL·min-1 and detection wavelength at 230 nm. Results The separation showed a good result. Conclusion The method is accurate and reproducible . It can be used for the quality control of Fengshigutong pills .%目的 建立高效液相色谱法测定风湿骨痛丸中双酯型生物碱的限度.方法 供试品采用超声处理法,双酯型生物碱中三种成分通过菲罗门C18被检测,柱温30℃,以乙腈-四氢呋喃-0.1 mol·L-1的乙酸铵溶液(每1 000 mL中加冰醋酸0.5 mL)(12.5:7.5:80)为流动相,检测波长230 nm.结果 乌头碱、次乌头碱、新乌头碱峰分离度均良好,且无干扰.结论 此法准确,重复性好,可用于控制该处方中制川乌、制草乌中双酯型生物碱的限度.