苯巴比妥联合 CCl4法建立肝硬化腹水大鼠模型

摘要

Objective To establish a stable rat model of liver cirrhosis by using carbon tetrachloride combined with Phenobarbital Sodi-um.Methods 35%Phenobarbital Sodium was carried out in SD rats for 7 days to activate hepatic microsomal enzyme P450.From the second week,gradient carbon tetrachloride oil solution was offered by intraperitoneal injection (twice per week for 14 weeks).24 h u-rine of rats was collected and measured at the end time of the trial.HE staining of liver tissue was used to observe pathological process of liver.Results After induced with Phenobarbital Sodium for 7 days and then injected with carbon tetrachloride into abdominal cavity continuously for 13 weeks,a stable rat model of liver cirrhosis could be achieved.Conclusion The procedure is stable and reliable for building liver cirrhosis model,and the model has lower mortality and shorter cycle time than traditional one.%目的：采用苯巴比妥联合四氯化碳构建稳定的肝硬化腹水大鼠模型，为抗肝硬化腹水药物的研究提供有效可靠的动物模型。方法采用苯巴比妥联合CCl4法复制肝硬化腹水大鼠模型。35％苯巴比妥溶液诱导1周，激活肝脏肝药酶P450活性，第二周开始，按梯度腹腔注射CCl4油溶液，每周2次至第14周。实验进行至中后期，收集大鼠24 h尿液，测量尿量；检查腹腔积液量；肝组织HE染色观察肝脏病理进程。结果大鼠经苯巴比妥溶液诱导1周后，连续腹腔注射CCl4油溶液13周，可复制稳定的肝硬化腹水模型。结论该法可建立稳定、可靠的肝硬化腹水模型，比传统模型降低了死亡率，且造模时间缩短。