冰王烧烫膏对大鼠深Ⅱ度烧伤的修复及机制研究

摘要

Objective To investigate repairing effect and mechanism of Ice king Burn-scald ointment on deep grade II burn wounds in rats.Methods Burned skin wound was made in 60 wistar male rats in accordance with the literature.The rats were randomly divided into 3 groups:the burn-scald ointment treated group, the jingwanhong ointment treated group, the model group, with 20 rats in each group.Samples were collected after 1,3,7,13,21 days of burn to determine healing rates and serum levels of TNF-αcontent.Expression changes of glut-2 receptor were detected by immunofluorescence 4 days after burn.Results There were no significant difference in the healing rates of the wound among the 3 groups after 1 day ( P>0.05) .The healing rates of the wound were significantly higher in the burn-scald ointment group and the model group (P<0.01) after 7,11,13,and 21 days.Compared with model group,glut-2 receptor expression was increased in the burn-scald ointment group,and the difference was statistically significant (P<0.01).Conclusion Ice king Burn-scald ointment shows a notable repairing effect on burn wound.The mechanism may be associated with decreased serum lev-els of TNF-αcontent and increased glut-2 receptor expression.%目的：研究探讨冰王烧烫膏对大鼠深Ⅱ度烧伤创面的修复及机制。方法雄性Wistar大鼠60只，制备深Ⅱ度烧伤创面的大鼠模型后，随机分为烧烫膏组、京万红组、烧伤模型组，每组20只。测量大鼠烧伤后1、3、7、13、21 d皮肤创面的愈合率和血清TNF-α含量；烧伤后4d免疫荧光法测glut-2受体的表达变化。结果烧伤后1 d各组创面愈合率比较，差异无统计学意义（P＞0．05），7、13、21 d烧烫膏能明显缩短烧伤大鼠皮肤创面愈合的时间，提高愈合率（P＜0．01）。烧伤后1、3、7、13、21 d烧烫膏组大鼠血清TNF-α水平呈明显下降，与模型组比较差异有显著性（P＜0．05，P＜0．01）。和模型组比较，烧烫膏组glut-2受体表达数目增加，差异有统计学意义（P＜0．01）。结论冰王烧烫膏对烧伤皮肤创面具有良好的修复作用并可能与降低血清TNF-α水平和增加glut-2受体表达有关。