西格列汀对肥胖大鼠血清内脏脂肪素水平的影响研究

摘要

目的 观察西格列汀对肥胖大鼠代谢指标及血清内脏脂肪素水平的影响.方法 40只SD大鼠随机分为普通饲料喂养组(ND组,n=10)和高脂饲料喂养组(HD组,n=30).16周后HD组成功建立肥胖模型(n=16),并随机分为肥胖对照组(OB组,n=8)和西格列汀干预组(SITA组,n=8).分别测定干预前后各组大鼠体质量(BW)、空腹血糖(FBG)、甘油三酯(TG)、总胆固醇(TCH)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、空腹胰岛素(FINS)及血清内脏脂肪素水平,计算胰岛素抵抗指数(HOMA-IR).结果 干预前,HD组的BW、FBG、TG、TCH、LDL-C、FINS、HOMA-IR、内脏脂肪素高于ND组,HDL-C低于ND组,差异有统计学意义(P<0.05).干预后,SITA组FBG、TG、TCH、LDL-C、FINS、HOMA-IR低于OB组,HDL-C高于OB组,差异有统计学意义(P<0.05).血清内脏脂肪素与BW、FBG、FINS、HOMA-IR、TCH、LDL-C呈正相关,与HDL-C呈负相关(P<0.05).结论 高脂饮食诱导肥胖大鼠模型出现糖脂代谢紊乱及胰岛素抵抗,血清内脏脂肪素水平明显增加,西格列汀能下调血清内脏脂肪素水平,改善糖脂代谢,减轻胰岛素抵抗.%Objective To observe the effect of sitagliptin on metabolic parameters ,serum visfatin level on high-fat-fed obesity rats. Methods Forty Sprague Dawley rats were randomly assigned into normal diet group (ND)(n=10)and high fat diet group(HD)(n=30). 16 weeks later,obese models(n=16)were established from HD group and were randomly assigned into obesity group (OB)(n=8)and sita-gliptin group(SITA)(n=8).Body weight(BW),fasting blood glucose(FBG),triglyceride(TG),total cholesterol(TCH),high-density lipo-protein(HDL-C),low-density lipoprotein(LDL-CC),fasting insulin(FINS),serum visfatin were measured before and after 6 weeks of treat-ment.Calculate HOMA-IR.Results The levels of BW,FBG,TG,TCH,LDL-C,FINS,HOMA-IR,serum visfatin in HD group were higher while the level of HDL-C was lower than those of ND group with a statistical significance (P<0.05).After treatment with sitagliptin,the lev-els of BW,FBG,TG,TCH,LDL-C,FINS,HOMA-IR,serum visfatin of SITA group were lower while the level of HDL-C was higher than those of OB group with a statistical significance (P<0.05).Pearson correlation analysis showed that serum visfatin was positively correlated with BW,FGB,FINS,HOMA-IR,TCH,LDL-C and negative correlated with HDL-C.Conclusion Obesity rat models induced by high-fat-fed show glucose and lipid metabolic disorders and insulin resistance .Serum visfatin levels are evaluated in obese rats , and sitagliptin intervention could lower serum visfatin levels in obese rats and improve the glucose and lipid metabolism and reduce insulin resistance .