MALDI-TOF-MS技术鉴定食源性致病菌的影响因素

摘要

Using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) to identify food-borne pathogens is still at a developing stage in China. The mass spectra obtained by MALDI-TOF-MS in identifying food-borne pathogens provides biological information of the strain and is the key to the successful identification. This article summarizes the main factors that influence the results of identification, including the selection of matrix, culture conditions of food-borne pathogens, method of sample preparation, and renewal and supplement of database, with a view to reducing the interference from these factors and giving full play to the advantages of MALDI-TOF-MS technique.%基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术鉴定食源性致病菌在我国尚处于发展阶段.MALDI-TOF-MS鉴定食源性致病菌形成的质谱图反映菌株的生物学信息,也是获得鉴定结果的关键.本文综述了影响MALDI-TOF-MS鉴定的主要因素:基质的选择、食源性致病菌的培养条件、样品的处理方式、数据库资源的更新与补充,以达到减小这些因素的干扰,最大程度地发挥MALDI-TOF-MS技术优势的目的.作为一种便捷的食源性致病菌鉴定技术,MALDI-TOF-MS必将在该领域发挥巨大作用.