蒙古黄芪的组织培养及辐射诱变研究

摘要

[Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of A.membranaceus Bge. [Method] With the different parts of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao aseptic seedling as explants (leaves, cotyledons, hypocotyls) induced callus, and cotyledon and hypocotyls taken by the method of radiation mutation were studied.[Result]The results showed that the three explants had relatively high callus induced rate in the medium which respectively made up of MS +6-BA 2.0 mg/L + NAA 2.0 mg/L, LS +6-BA 2.0 mg/L +NAA 0.1 mg/L, MS + 6-BA 2.0 mg/L + NAA 2.0 mg/L; the optimum mutation time of hypocotyls and cotyledons was 15 minutes; the growth of the callus induced from hypocotyls would be better as the mutation time increased, but when it reached a certain time the growth would be weaken, the induction rate also would be reduced. [Conclusion] This study will provide the scientific reference in tissue culture and mutation breeding of A.membranaceus Bge.