SSR分子标记技术研究紫菜薹资源的亲缘关系

摘要

[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers selected from cabbage primers, and the cluster analysis was carried out. [Result] A total of 23 pairs of SSR primers were screened; cluster analysis showed that the genetic relationship of purple tsai-tai germplasms had relatively significantly locality, and the 45 purple tsaitai samples can be divided into three groups of Sichuan, Hunan and Hubei; furthermore, the similarity coefficients of 45 purple tsai-tai samples were all greater than 0.5 （ranging from 0.547 0 to 0.910 7）, indicating that the closer the genetic relationship among purple tsai-tai samples is, the narrower the genetic basis will be. [Conclusion] This study provides a theoretical and technical basis for the identification, protection and breeding of the resources of local varieties of seaweed sprouts. This study provided theoretical and technical basis for the identification, protection, breeding and utilization of local purple tsai-tai resource.%[目的]利用SSR分子标记技术研究紫菜薹资源的亲缘关系。[方法]用从大白菜引物中筛选出的65对引物，对收集到的45份紫菜墓地方资源进行SSR分析，并对结果进行聚类分析。[结果]试验共筛选出23对SSR引物；聚类分析表明，紫菜薹亲缘关系表现出较为明显的地域性，大致可分为四川、湖南、湖北3个类群；同时，45份资源的相似系数都大于0．5（介于0．5470-0．9107之间），表明紫菜薹各品种间亲缘关系较近，遗传基础较为狭窄。[结论]该研究为紫菜薹地方品种资源的鉴定、保护以及育种利用提供了理论和技术依据。