60Co-γ射线辐照陆地棉花粉后对M1代农艺性状的影响．

摘要

[Objective] The aim was to provide reference for research on radiation and breeding of cotton pollen through irradiating common ripe pollen grain of upland cotton by 60Co-γ Ray of varied doses. [Method] Ripe pollen grains of upland cotton were irradiated by 60Co-γ Ray with doses of 5, 10, 15, and 20 Gy, respectively, to learn radiation effect and select appropriate dose. [Result] Most properties of M1 obviously showed variation when dose was over 10 Gy; vitality, growth, and fertility were greatly inhibited when dose was 15 Gy which was almost semi-lethal concentration, and variation species were richest at the same time, which provided materi- als for practical breeding. [Conclusion] 60Co-γ Ray of 15 Gy is more suitable for mutagenesis research on ripe pollen grains of upland cotton.%[目的]利用不同剂量的60Co-γ射线辐照一陆地棉常规栽培种成熟花粉粒，为棉花花粉辐射育种研究提供参考。[方法]分别利用剂量为5、10、15、20Gy的60Co-γ射线对陆地棉成熟花粉粒进行辐照处理，了解辐射效应，筛选适宜剂量。[结果]当剂量大于10Gy时，M1大部分，性状表现出明显变异，剂量为15Gy时对棉花当代的生活力、生长势、育性等抑制作用最大，基本为半致死剂量，但田间出现的变异类型也是最丰富的，有利于为实际育种选育材料。[结论]剂量为15Gy的60Co-γ射线比较适宜于陆地棉成熟花粉粒的诱变研究。