不同果桑品种雌花差异表达蛋白质的分离与鉴定

摘要

[Objective] This study aimed to explore the proteins related to pistil ate flower development in different mulberry cultivars. [Method] The total proteins of the pistil ate flowers of two mulberry cultivars Da10 (Morus atropurpurea Roxb.) and SG01 (Morus multicaulis Perr.) were extracted, separated and detected through two-dimensional electrophoresis (2-DE) and mass spectrometry. [Result] There was sig-nificant difference in the expression of proteins from the pistil ate flowers of different mulberry cultivars. From the 2-DE images of Da10 and SG01, 445±17 and 425±12 protein spots were respectively detected. The expression levels of 75 protein spots differed significantly. Thirteen spots those were expressed at high levels and wel separated were analyzed by mass spectrometry, and nine of them were identified successful y. The nine proteins are involved in the glycometabolism, protein and amino acid metabolism and defense responses during the development of mulberry pistil ate flower after they were pol inated. [Conclusion] The findings wil provide refer-ence for further study on the molecular mechanism of mulberry pistil ate flower de-velopment.%[目的]探讨不同果桑品种雌花发育相关蛋白质的表达特征。[方法]以果桑品种“大10”和“SG01”为材料，采用双向电泳和质谱技术对授粉后雌花蛋白质组的差异表达进行分离与鉴定。[结果]在不同果桑品种间，授粉后雌花蛋白质的表达存在较大差异，从“大10”与“SG01”雌花穗蛋白质的2-DE图谱中分别检测到了445±17和425±12个蛋白质斑点，其中表达量差异达2倍及以上的斑点共有75个。从中选取13个表达量较为丰富且分离效果良好的蛋白斑点进行质谱分析，结果成功鉴定了其中的9个，这些蛋白质组分分别参与了桑雌花发育过程中糖代谢、蛋白质及氨基酸代谢以及防御应答反应等生命活动。[结论]该研究为深入研究桑雌花发育的分子机理奠定了基础。