抗草甘膦野生大豆筛选及其抗性生理机制研究

摘要

[目的]初步确定抗草甘膦野生大豆抗性产生的生理机制，为抗草甘膦大豆育种奠定基础。[方法]利用田间试验对67份野生大豆材料进行草甘膦抗性筛选；并以抗性材料ZYD0685和敏感材料 ZYD0790进行生理指标研究。[结果]在草甘膦有效剂量为1.23 kg/hm2条件下，不同野生大豆对草甘膦抗性存在明显差异，其中 ZYD0685和 ZYD2405的存活率最高，分别为87%和83%；7份材料的存活率为2.7%~38%；58份材料死亡。在草甘膦不同剂量处理下，抗性材料 ZYD0685叶绿素和莽草酸含量没有明显变化，GSTs活性明显增加；而敏感材料 ZYD0790莽草酸含量明显升高，叶绿素含量明显降低，GSTs活性变化幅度较小。[结论]降低莽草酸积累量较少是野生大豆抗草甘膦的主要生理反应。%[Objective] This study aimed to preliminarily determine the physiological mechanism of glyphosate resistance produced in wild soybean(Glycine soja) and fur-ther provide a basis for the breeding of glyphosate-resistant soybean. [Method] First, a screening for glyphosate resistant varieties among sixty-seven wild soybean mate-rials was done in a field trial; subsequently, physiological indexes of the screened resistant variety ZYD0685 and the sensitive variety ZYD0790 were studied. [Result] At the glyphosate dose of 1.23 kg a.i/hm2, glyphoaste resistance varied greatly a-mong different wild soybean materials, with the highest survival rate of 87% and 83% occurring in ZYD0685 and ZYD2405, respectively, and that of another seven accessions ranged from 2.7% to 38%, and al the remaining fifty-eight soybean ma-terials died. After treatment with glyphoaste at different doses, there were no signifi-cant differences in chlorophyl content and shikimate content in the resistant ZYD0685, but there was an evident increase in the activity of gultathione-S-transferas-es(GSTs); while in the sensitive ZYD0790, the content of shikimic acid increased sig-nificantly, and chlorophyl content decreased significantly, and GSTs activity revealed a slight change. [Conclusion] Therefore, lowering the amount of accumulated shikimic acid is the major physiological response to glyphosate in wild soybean.