乙醇等有机溶剂对肿瘤细胞PC12的毒性剂量分析

摘要

The morphologic changes and growth status of PC12 cells were observed after intervened by different concentrations of methanol, ethanol, acetone, glycerol and the toxic concentrations were ascertained. Four kinds of organic solvents al showed certain cytotoxicity to PC12 cells. Compared with other three kinds of or-ganic solvents, ethanol showed the most obvious cytotoxicity to PC12 cells and the cellviability would be reduced to 60% if the concentration of ethanol was 20 ml/L and the intervention lasted for 24 h. Under the same condition, the reduced per-centages of cellviability for acetone and ethanol were 20% and 15% respectively. Glycerol also showed cytotoxicity to PC12 cells, especial y as the concentration was raised gradual y, but the toxicity was relatively mild. This study would provide refer-ence material for subsequent pharmacological studies.%分析一定浓度的甲醇、乙醇、丙酮和甘油作用下肿瘤细胞 PC12形态和生存率的变化，确定上述有机溶剂对细胞的毒性剂量。研究发现，PC12细胞对上述溶剂表现出不同的耐受性，其中乙醇的毒性剂量最低，20 ml/L浓度作用24 h细胞生存率就下降近40%；该浓度的丙酮和甲醇干预 PC12细胞24 h对应的细胞生存率则分别下降约20%和15%；甘油的作用相对缓和，但随着干预浓度逐渐增大时，也表现明显的细胞毒性。该研究可为后续药理研究提供参考资料。