定向转育卵圆生态型大白菜雄性不育系的研究

摘要

[目的]探究大白菜定向转育卵圆生态型雄性不育系的方法。[方法]以大白菜核不育"复等位基因遗传假说"为理论依据，以甲型两用系‘AB12’为不育供体品系，以卵圆生态型可育品系‘06048’为待转育品系，采用杂交、回交、兄妹交和测交的方法，按照定向转育方案将核不育基因定向转育。[结果]各世代育性分离比率与理论相一致，成功育成了一种不育株率100%的具有‘06048’园艺性状的新核不育系，实现了核不育基因和园艺特性的同时转育。[结论]该研究成果验证此定向转育方案是可行的，进一步为基因型为 msms的其他园艺性状的大白菜定向转育提供理论基础，也可以推广应用到整个芸薹属其它经济作物中，极大的拓宽了优良核不育基因的实际应用范围。%Objective] The study aimed to explore the method for directional breeding of a male-sterile line in oval-ecotype Chinese cabbage. [Method] Based on "Multiple Al ele Hypothesis of Genic Male Sterile Chinese Cabbage", an inbred line ‘06048’ of oval ecotype was used as the receptor, and male fertile plant of ‘AB12’ was used as the donor line. Crossing, backcross, selfing, testcross and sibling were ap-plied to transfer the multiple al eles under the directional genetic model. [Result] Segregation ratio of every generation was consistent with theoretical value. A new male sterile line with 100% male sterility and ‘06048’ horticultural traits was ob-tained successful y, which accomplished the transfer of male sterile multiple al ele and horticultural characters of receptor line at the same time. [Conclusion] The re-search verifies that the model of directional transfer is feasible, provides a theoreti-cal basis for the directional transfer of Chinese cabbage with other horticultural traits whose genotype is msms. The model can also be applied to other Brassica crops, to generate genetic male sterile lines with specific botanical traits and high-quality economic traits.