Verification of the utility of molecular markers linked to the multiple-allele male-sterile gene Ms in the breeding of male-sterile lines of Chinese cabbage (Brassica rapa)

摘要

To verify the molecular markers linked to the genic multiple-allele male-sterile geneMs, an F1?plant, which was generated by crossing the inbred line a20 and the male-sterile plant of the genic multiple-allele male-sterile AB line, was backcrossed with an a20 plant to develop BC4?and BC5?populations. Sequence-characterized amplified region (SCAR) marker?syau_scr01?and simple sequence repeat (SSR) marker?syau_m13, which were linked to?Ms, exhibited polymorphism between the 2 parents. The accuracies of these 2 markers in determining the plant genotype was 85 and 91.7%, respectively. The accuracy reached 100% when the 2 markers were used in combination. These results indicate that these 2 markers can be applied in the marker-assisted selection of the genic multiple-allele male-sterile line of Chinese cabbage.