不同引物鉴定同一杂交水稻种子样品纯度结果的差异及其原因分析

摘要

在对冈优158、Ⅱ优808、五优308及天丰优316的杂交种子样品进行室内纯度鉴定时，发现不同 SSR引物鉴定的纯度结果存在明显差异。为了探讨其原因，将这4个组合的种子样品在海南进行田间种植鉴定，并按单株进行了分子与田间表型的对比分析。结果表明，冈优158、Ⅱ优808、五优308和天丰优316分别用引物 RM208、RM264、RM242和RM164鉴定种子纯度时，只能鉴定出不育系（母本）杂株，室内分子鉴定纯度的结果均高于相应的田间纯度鉴定结果；而引物 RM341、RM297、RM21和RM110不仅能鉴定出不育系（母本）杂株，还能鉴定出串粉株，室内分子鉴定纯度与田间鉴定纯度相当。因此，室内纯度鉴定时应采用多对引物进行检测，以增加识别串粉株的几率。%Different results of seed purity identification for Gangyou 158, ⅡYou 808, Wuyou 308 and Tianfengyou 316 were obtained using different SSR primers in our early work. To find out the reasons, the four hybrid combinations were grown in field to identify their purity according to their phenotypic traits. Then, the results of field identification were compared with that of laboratory tests using different SSR primers. The comparison revealed that only sterile lines (female parent) were distin-guished from true hybrids using the primers RM208, RM264, RM242 and RM164 for the purity identification of Gangyou 158, ⅡYou 808, Wuyou 308 and Tianfengyou 316, so the results were higher than that of field identification. In contrast, the primers RM341, RM297, RM21 and RM110 were able to distinguish not only the sterile plants but also the cross-pol inated ones from the true hybrids of Gangyou 158, ⅡYou 808, Wuyou 308 and Tianfengyou 316, and the results of purity identifi-cation using them were close that of field identification. In summary, several pairs of primers should be used for the purity identification of rice hybrids to distinguish al the off-type plants and thus improve the accuracy.