笃斯越橘新品种'紫水晶'离体培养关键技术研究

摘要

Objective] This study aimed to discuss the key technology of in-vitro cul-ture for a new Vaccinium uliginosum cultivar Zishuijing. [Method] Through the screen-ing and optimization of sterilization method for explants, sampling time, multiplication, nursing and rooting culture, a matching clone propagation system was established for the new Vaccinium uliginosum cultivar Zishuijing. [Result] The explants were sterilized with 0.1% HgCl2 for 3 min; the differentiation and multiplication medium of Zishuijing was composed of WPM (modified), 6-BA (1.0 mg/L) and ZT (1.0 MG/L); the rootless tube seedlings were transplanted in organic matrix (sawdust∶bark∶peat=1∶1∶1) in September and cultured at air relative humidity of 80%-90% and temperature of 20-25 ℃, and after 50 d, the rooting rate reached 72.4%. [Conclusion] The key technol-ogy of in-vitro culture for the new Vaccinium uliginosum cultivar Zishuijing was estab-lished, thereby providing technical support for large-scale industrialized seedling pro-duction of Zishuijing.%[目的]探讨笃斯越橘新品种"紫水晶"离体培养的关键技术。[方法]通过对外植体灭菌方法，取材时期，增殖、壮苗、生根培养的筛选与优化，建立适合笃斯越橘新品种"紫水晶"的无性配套繁殖体系。[结果]外植体灭菌采用0.1% HgCl2消毒3 min；WPM（改良）+6-BA 1.0 mg/L + ZT 1.0 mg/L为笃斯越橘新品种"紫水晶"分化增殖培养基；9月份将无根试管苗移栽到混配有机基质锯末∶树皮∶草炭=1∶1∶1基质中，空气相对湿度80%~90%，培养温度为20~25℃，50 d后生根率达72.4%。[结论]建立了笃斯越橘新品种"紫水晶"离体培养关键技术，为大规模工厂化育苗提供技术支持。