纳米金属材料在红掌农杆菌介导法中抑菌效果的分析

摘要

[目的]探讨纳米金属材料应用于红掌遗传转化中抑制农杆菌污染的新技术.[方法]分别在农杆菌培养基和红掌愈伤组织培养基中添加不同种类的纳米金属材料,调查其对农杆菌生长、愈伤组织生长及分化、农杆菌污染抑制效果的影响.[结果]在供试的四种纳米金属材料中,纳米载银试剂对农杆菌生长有显著的抑制作用,其最低抑菌浓度为25 mg/L;在红掌转基因材料受到农杆菌污染的情况下,采用25 mg/L的纳米载银试剂处理ld,抑菌效果良好,抑菌率可达100％,并且对红掌愈伤组织生长及再分化无不良影响.[结论]纳米载银试剂能够有效抑制农杆菌污染,其抑菌效果显著优于头孢霉素和羧苄青霉素.%[Objective] This study was conducted to investigate the application of nanometallic materials in inhibiting Agrobacterium contamination in genetic transformation of Anthurium.[Method] Different nanometallic materials were added into Agrobacterium medium and Anthurium callus medium,to investigate the effects of their effects on Agrobacterium growth,callus growth and differentiation,and Agrobacterium contamination.[Result] Among the 4 nanometallic materials,NanoAg-2 showed a significant inhibitory effect on the growth of Agrobacterium,with a minimal inhibitory concentration of 25 mg/L.Even for the Anthurium calli or transgenic material contaminated by the Agrobacterium,a good antibacterial effect could be achieved after treating with 25 mg/L NanoAg-2 for 1 d with oscillation,the antibacterial rate reached 100％,and the Anthurium calli could grow and differentiate normally.[Couclusion] NanoAg-2 could effectively inhibit Agrobacterium contamination,and its antibacterial effect is significantly better than cephalosporin and carbenicillin.