青岛泊子盐场放线菌多样性及其功能酶的筛选

摘要

In this study ,15 actinomycetes were isolated using three different media (M1~M3 ) from a salt pond of Qingdao .Bioinformatics analysis of the 16S rRNA sequences from the isolates revealed they belonged to three genera (Streptomyces ,Nocardiopsis and a novel genus) ,with Streptomyces being the dominant genus .The strain CXB832 were 95 .4 % and 94 .9 % homologous to the two standard strains of bacteria ,and therefore was candidates for a new genus on the basis of phenotypic ,chemotaxonomic and phylogenetic distinctiveness .The type of medium and temperature showed significant effects on the number and diversity of actinomycetes recovered .Starch casein medium was the most effective media to isolate actinomycetes species at 37℃ .12 ,3 ,2 and 1 of 15 isolates produces amylase ,lipase ,caseinase and cellulose ,respectively .%采用平板涂布法利用3种培养基从青岛即墨市田横镇泊子盐场盐池底泥样中共分离纯化出15株放线菌，对分离菌株进行16S rRNA基因测序分析。发现这15株放线菌分属于链霉菌属（ Streptomyces ）、拟诺卡氏菌属（Nocardiopsis）和1个新属，其中 Streptomyces属为优势菌属；菌株CXB832与 Nocardiopsis arabia DSM 45083T和 Haloacti-nospora albus DSM 45015T最接近，同源性分别为95．4％和94．9％，根据其表型和分子特征可以初步判定该菌株为放线菌新属。培养基及培养温度对盐池环境中放线菌的分离效果均有影响，利用淀粉酪素培养基在37℃分离的放线菌种类和数量较多。在15株放线菌中，分别有12株、3株、2株和1株放线菌产淀粉酶、酯酶、蛋白酶和纤维素酶。