四溴双酚 A（TBBPA）对叉鞭金藻的毒性效应研究

摘要

以叉鞭金藻(Dicrateria inornata )为研究对象,分析了不同质量浓度四溴双酚 A(TBBPA)污染下微藻光合色素含量、可溶性蛋白含量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,以及细胞形态和细胞内部结构的变化。研究结果显示：在 TBBPA 较低质量浓度(0.5和2.0 mg/L)污染条件下,叉鞭金藻的叶绿素 a(Chl-a)、叶绿素 c (Chl-c)和类胡萝卜素(Car)含量显著高于对照组,其含量第24小时达最大值；较高质量浓度(4.0和8.0 mg/L)污染条件下,3种色素含量则显著低于对照组,其含量在第24小时达最小值。实验期间各实验组叉鞭金藻的可溶性蛋白含量、SOD 活性和 MDA 含量主要被诱导；4.0 mg/L 的 TBBPA 作用叉鞭金藻96 h 后,藻细胞表面形态和内部亚显微结构均发生变化,与光合色素含量降低、MDA 含量增加相一致。%To study the toxic effect of Tetrabromobisphenol A (TBBPA)on marine microalgae,Dicrateria inornata was exposed to five different concentrations (0,0.5,2.0,4.0 and 8.0 mg/L)of TBBPA.The contents of photosynthetic pigments,soluble protein,and Malondialdehyde (MDA),and activities of Su-peroxide Dismutase (SOD)were monitored within 96 hours.In addition,the cellular morphology and sub-structure were observed after exposure 96 h.The results showed that three pigments (chlorophyll a and c , and carotene)in lower-concentration groups (0.5 and 2.0 mg/L)were obriously higher than those of the control,and their contents reached highest values at 24 h.While they were remarkably lower than those of the control in higher-concentration groups (4.0 and 8.0 mg/L),and their contents showed lowest values at 24 h.Significant induction was observed in most cases in all the test groups for soluble protein content, SOD activity,and MDA content.MDA content increased with increasing of TBBPA concentration at 96 h. The changes of cellular morphology and substructure were observed after exposure of high concentration TBBPA (4.0 mg/L),which was consistent with the decrease of photosynthetic pigments contents and the increase of MDA contents.