平衡针对腰椎神经根压迫模型大鼠血浆β-内啡肽、促肾上腺皮质激素的影响

摘要

Objective To observe the effect of balance acupuncture on the thermal pain threshold and plasma β-endor-phin (p-EP) and adrenocorticotrophic hormone (ACTH) levels in lumbar disc herniation rats so as to research its mechanisms underlying improvement of lumbar disc protrusion. Methods A total of 120 male rats were randomly divided into blank control (control) group, model group. Sham-operation(sham)group, and acupuncture group (n = 30) which were further divided into 7-days (d) and 14-d subgroups. Rats in the acupuncture group were treated with balance acupuncture of "Back Pain" point (the midpoint between two eyes) and "Hip Pain"point (the midpoint between the acromial process and the axillary furrow). Thermal pain threshold was detected by using radiant heat detector. Plasma p-EP and ACTH levels were determined by radioimmunoassay. Results Compared with the control group and pre-operation and sham group, the thermal pain reaction was increased significantly in the model group (P<0. 05). In comparison with the model group, the thermal pain reaction was decreased obviously from day 10 after acupuncture treatment in the acupuncture group (P<0. 05). Compared to the control group, plasma p-EP contents on day 7 and 14 and ACTH level on day 14 in the model group were increased significantly (P<0. 05) .while in comparison with the model group, plasma P-EP contents on day 7 and 14 and ACTH level on day 14 in the acupuncture group were down-regulated markedly (P<0. 05). No significant difference were found between the control and sham groups in plasma P-EP contents and ACTH levels on day 7 and 14 (P>0. 05). Conclusion Balance acupuncture treatment can lower plasma ACTH level in lumbar disc herniation rats, which may contribute to its effect in easing lumbar disc herniation pain in clinic practice.%目的:初步探求平衡针治疗腰椎间盘突出神经根压迫症状的作用机制.方法:将SD雄性大鼠随机分为空白组、假手术组、模型组、治疗组,每组30只,各组中又分为7d取材组和14d取材组各15只.用肠线环扎L4神经根造模.治疗组针刺“腰痛穴”“臀痛穴”,每日2次.用热痛测试仪检测各组大鼠造模前及造模后第4、7、10、14天的缩爪潜伏期,采用放射免疫分析法测定血浆β-内啡肽(EP)及促肾上腺皮质激素(ACTH)的变化.结果:模型组大鼠痛敏现象明显增强(P＜0.05),血浆β-EP、ACTH的含量呈增高趋势；经过平衡针治疗,治疗组痛敏现象至治疗第10天起较模型组明显降低(P＜0.05),血浆中β-EP的含量第7天时就较模型组显著降低(P＜0.05),血浆中ACTH含量第14天时显著低于模型组(P＜0.05).结论:平衡针有很好的减轻疼痛作用,其机制可能是通过影响ACTH分泌代谢而调节机体应激状态,而是否通过调节β-EP分泌代谢发挥作用尚需进一步增设时间点观察.