电针对氯胺酮成瘾大鼠海马CA1区酪氨酸羟化酶、C-fos表达的影响

摘要

目的:探讨电针对氛胺酮滥用成瘾戒毒治疗的物质基础.方法:将40只SD大鼠随机分为正常对照组、生理盐水(10mL/kg)对照组、氛胺酮(100mg/kg)组、氯胺F1(100mg/kg)加电针组.按上述设定剂量,经腹腔注射给药,每天1次,连续7d,氯胺酮加电针组于给药1周开始低频(2Hz)电针交替刺激双侧"足三里"与"三阴交"穴位,每次30min,每天1次,连续1周.采用免疫组织化学染色方法显示大鼠海马CA1区酪氨酸0化酶(TH),c-fo、的表达.结果:与正常对照组和生理盐水对照组比,氛胺酮组大鼠海马CA 1区TH,c-fos免疫反应阳性神经元的数目和阳性产物的表达水平明显增强(P<0.05,P<0.01).与氯胺酮组比,氛胺酮加电针组TH,c-fo:免疫反应阳性神经元的数目和阳性产物的表达显著减弱(P<0.01).结论:电针"足三里","三阴交"可明显抑制氛胺酮成瘾引起的海马CA 1区TH,C-foS表达的增强,提示电针可能通过抑制海马多巴胺神经元的活动改善氛胺酮成瘾.%Objective To explore the morphological basis of electroacupuncture (EA) in relieving addiction of ketamine.Methods Forty Sprague-Dawley rats were randomized into normal control, normal saline ( 10 mL/kg), model and ketamine+ EA groups.Ketamine-addiction model was established by intraperitoneal administration of ketamine (100 mg/kg), once a day for 7 days.EA (2 Hz) was applied to "Zusanli" (ST 36) and "Sanyinjiao"(SP 6) for 30 min, once a day for 7 days.The expression of tyrosine hydroxylase (TH) and c-fos in the hippocampal CA 1 region was detected by immunohistochemistry.Results In comparison with the normal control group and normal saline group, the numbers of both TH immuno-reaction (IR)-positive and cfos IR-positive neurons in the hippocampal region in the model group were increased significantly (P＜0.05, P＜0.01).Correspondingly, the expression of both TH IR-positive and c-fos IR-positive products in the hippocampal CA 1 region in the model group was upregulated evidently ( P＜0.05, P＜0.01 ).After EA of "Zusanli" (ST 36)-"Sanyinjiao" (SP 6), compared with the model group, the expression of both TH IR-positive and c-fos IR-positive products was downregulated considerably (P＜0.01).Conclusion EA of "Zusanli"(ST 36)-"Sanyinjia"(SP 6) can downregulate ketamine-addiction induced increase of expression of tyrosine hydroxylase and c-fos in the hippocampal CA 1 region in ketamine-addiction rats, which may contribute to its effect in relieving ketamine addiction symptoms in clinic.