Objective To investigate the influence of electroacupuncture (EA) on lipid metabolism in both sexes of o-besity model rats. Methods A total of 30 male and 30 female SD rats were randomized into normal control, model and EA groups respectively, with 10 rats in each group. EA (2 Hz, 1-2 mA) was applied to the ipsilateral "Zusanli" (ST 36>-"Sanyin-jiao"(SP6) and bilateral'Tenglong" (ST40) for 30min, once daily for 28 days. In addition,"Quchi"(LI 11) ,"Zhongwan"(CV 12) and"Guanyuan"(CV 4) were punctured with filiform needles and stimulated by manipulating the needle for 1 min, separately. Obesity model was duplicated by subcutaneous injection of 15% glutamic sodium (0. 2 mL/10 g) once daily for 5 days and by feeding the animal with high fat forage for 36 days. Lee's index was calculated, and plasma triglyeride (TG) concentration detected by glycerol-phosphoric acid oxidase-peroxydase method, total cholesterol (TO detected by cholesterol oxidase method, and plasma low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) contents were assayed by one-step method, respectively. Results In both male and female rats, compared to their own normal groups, the Lee's index, plasma TG, TC and LDL-C contents were increased significantly and plasma HDL-C contents decreased obviously in the model groups (P<0. 01), while compared to their own model groups, the Lee's index, plasma TG. TC and LDL-C levels in the EA groups were down-regulated considerably and plasma HDL-C contents up-regulated remarkably after the treatment ( P<0. 01, P<0. 05) . The efficacies of the EA intervention were obviously better in reducing Lee's index for male rats, and in down-regulating plasma TG and TC contents in female rats (P<0. 05). No significant differences were found between male and female rats in EA intervention for plasma LDL-C and HDL-C levels Conclusion EA intervention can effectively regulate obesity rats' Lee's index and plasma TG, TC. LDL-C and HDL-C levels. The effects are different in male and female rats.%目的:探讨电针刺激对雌雄两性肥胖大鼠脂代谢影响的差异及其作用机制.方法:SD大鼠随机分为模型雌组、模型雄组、电针雌组、电针雄组,并设正常雌组和正常雄组进行对照.采用谷氨酸钠和高脂饮食诱导的下丘脑性肥胖模型.电针雌组、雄组电针大鼠“后三里”“三阴交”“丰隆”等穴.计算各组治疗前后Lee's指数,生化法检测各组血浆甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)含量.结果:电针雌、雄两组的Lee's指数及血浆TG、TC、LDI-C含量均较模型雌、雄两组明显降低(P＜0.01,P＜0.05),而血浆HDL-C含量均有明显升高(P＜0.05).电针雄组Lee's指数较电针雌组明显降低(P＜0.05)；电针雌组血浆TG、TC含量较电针雄组明显降低(P＜0.05)；电针雌组血浆HDL-C、LDL-C的含量与电针雄组比较差异无统计学意义(P＞0.05).结论:电针刺激对雌雄两性肥胖性大鼠均有不同程度的减肥作用,但在降低Lee's指数和调节脂代谢方面存在着一定的差异,电针可更明显地降低雄性大鼠的Lee's指数及雌性大鼠的血浆TG、TC含量,但对雌雄两性血浆HDL-C、LDL-C含量的影响无明显差异.
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