RGD多肽纳米纤维凝胶的自组装及其与脂肪干细胞生物相容性

摘要

To synthesize bioactive Arg-Glu-Asp( RGD ) peptide and self-assemble in vitro and make a pilot study of its biocompatibility with adipose derived stem cells. Methods RGD peptide was synthesized by solid-phase peptide synthesis technique and induced self-assembly with DMEM containing 0. 5 mol/L Ca2 + . The morphological features of the assembled material were studied by tansmission electron microscope( TEM ). The adipose derived stem cells( ADSCs ) were co-cultured with RGD peptide nanofiber hydrogels. Calcein-AM/PI staining was used to show the percentage of live cells and CCK-8 kit was used to test the effect of RGD peptide nanofiber hydrogels on proliferation of ADSCs. Results TEM showed the self-assembled gel was nano-fibers with diameter of 10nm and length of hundreds nanometers. There were no obvious negative effect on proliferation of ADSCs. Conclusion RGD peptide can self-assemble to nano-fiber gel,which has good biocompatibility.%目的 研究精氨酸-丙氨酸-天门冬氨酸(RGD)多肽纳米纤维凝胶在体外的自组装及其与脂肪干细胞的生物相容性.方法 固相法合成含RGD序列的多肽,加入含0.5 mol/L Ca2+的DMEM诱导其自组装,透射电镜观察自组装效果.将其与体外分离培养的大鼠脂肪干细胞复合培养,倒置显微镜下观察细胞的生长情况,Calcein-AM/PI染色计数活细胞比例,CCK-8试剂盒检测RGD多肽纳米纤维凝胶对大鼠脂肪干细胞增殖的影响.结果 RGD多肽在Ca2+诱导下可自组装形成水胶体,透射电镜观察其微观结构为直径10 nm左右的纳米纤维,长度可达数百纳米.对大鼠脂肪干细胞生长增殖未见负面影响.结论 RGD多肽纳米纤维凝胶具有良好的生物相容性.