METHOD OF PRODUCING THREE DIMENSIONAL AUTOLOGOUS FAT GRAFT USING HUMAN LIPOASPIRATE-DERIVED ADIPOSE TISSUE WITH MULTIPOTENT STEM CELLS AND BIOCOMPATIBLE CELLULOSE NANOFIBRILS

摘要

Autologous 3D fat grafting and modification of adipose tissue lipoaspirate rich in stem cells with biocompatible cellulose nanofibrils (CNF) or collagen nanofibrils to achieve processable dispersion/emulsion which can be formed into a 3D shape(s) with desired porosity. 3D construct formed is further crosslinked either in situ at the implantation site or before implantation. Grafting can be performed in human or animal to correct soft tissue contour deformities in reconstructive and aesthetic surgery, including but not limited to injection, moulding, or 3D bioprinting. Biocompatible cellulose or collagen nanofibrils act as a dispersant/emulsifier of disintegrated lipoaspirate and provide shear thinning properties which make it possible to process dispersion into 3D shape yet provide porosity for nutrient diffusion and neovascularization. Invention further comprises a crosslinker(s) to provide construct's mechanical stability and ability to be transplanted as larger structure.