硝苯地平对人肝星状细胞系-LX2活化及分泌的影响

摘要

Objective To investigate the effect of nifedipine( NF ) on the regulation of proliferation and function of human hepatic stellate cell( HSC ) lines. and to illuminate its further pharmacological role. Methods Activation of HSC culture system stimulated by IFN-γ was established in vitro .which was separated three groups : control, IFNγand IFN-γ + NF. The control group was added medium only. The IFN-γ group was added medium and IFN-γ. The IFN-γ + NF group was added medium, IFN-γ and NF. Slide of crawling cell was adopted to identify morphology of HSC cell. Flow cytometer was employed to detect the activation of HSC by analyzing the level of a-SMA. ELISA was used to determine the concentration of TGF-β. Results Compared with the control group, IFN-γ could induce the expression of a-SMA and TGF-β of HSC in vitro( P ＜0. 05 ). However. NF could inhibit the expression of α-SMA and TGF-β of HSC( P ＜ 0. 05 ). Conclusion NF can inhibit the proliferation and activation of HSC which suppress the occurrence of liver fibrosis.%目的 探讨硝苯地平(NF)对人肝星状细胞系(HSC)-LX2细胞的活化及分泌转化生长因子(TGF)-β的影响.方法 体外建立干扰素(IFN)-γ刺激HSC活化的培养体系,设3组:对照组、IFN-γ组和IFN-γ+NF组,对照组仅加入培养基,IFN-γ组加入IFN-γ和培养基,IFN-γ+NF组加入IFN-γ、NF和培养基,于0、1、2、3、5 d观察HSC-LX2活化增殖和分泌细胞因子的能力,采用细胞爬片和免疫组化技术鉴定活化HSC的细胞形态,流式细胞术分析HSC表达α-平滑肌肌动蛋白(α-SMA)水平,ELISA法测定不同组HSC分泌TGF-β的浓度.结果 与对照组比较,IFN-γ组HSC活化和分泌TGF-β的能力较强,差异有统计学意义(P<0.05);与IFN-γ组比较,IFN-γ+NF组HSC活化和分泌TGF-β的能力较弱,差异有统计学意义(P<0.05).结论 NF体外实验中能抑制HSC-LX2的增殖和活化,具有潜在的抗纤维化作用.