酶联免疫斑点试验诊断结核的敏感性和特异性研究

摘要

To detect Mycobacterium tuberculosis antigen specific T cells in blood samples with T -SOFT· TB assay from 106 patients with active tuberculosis and 24 healthy individuals , and to compare with that from PPD test, acid-fast stain and tuberculosis antibody test. T-SOPT· TB showed a sensitivity of 93. 4% and specificity of 95. 8%. While the sensitivity from tests of AFB , PPD and tuberculosis antibody were 36. 8% , 57. 5% and 39. 6% , and the specificity was 100.0%, 83.3% and 91.7% respectively. Comparing with the other three methods, sensitivity difference in T-SOPT · TB for diagnosing tuberculosis infection was statistically significant ( P < 0. 01). While the difference in specificity was no statistically significant. As a result, T-SOPT · TB assay is a sensitive and specific method for detecting Mycobacterium tuberculosis infection. It will be a useful tool to diagnose tuberculosis infection early and rapidly.u0000fer%应用T-SPOT·TB检测临床确诊的106例结核患者和24例健康体检者,与抗酸染色(AFB)、结核菌纯蛋白衍生物试验(PPD)、结核抗体检查结果 进行比较分析.结果 显示,T-SPOT·TB敏感性为93.4%,特异性为95.8%.AFB、PPD、结核抗体敏感性分别为36.8%、57.5%、39.6%,特异性分别为100.0%、83.3%、91.7%.T-SPOT·TB敏感性与三者相比,差异有统计学意义(P＜0.01),特异性与三者相比,差异无统计学意义.表明酶联免疫斑点T-SPOT·TB实验检测结核敏感性强、特异性高,是快速诊断结核感染的有效手段.