安徽汉族人群IL-1F10-3基因rs3811058位点单核甘酸多态性与强直性脊柱炎患者遗传易感性研究

摘要

To investigate the expression and clinical significance of acid phosphatase ( ACP ) in the occurrence of cervical carcinoma. Methods RT-PCR, Western blot and immunohistochemical method were used to detect the expression levels of ACP in four groups: 30 cases of normal cervical tissues,22 cases of CIN I ~ II ,22 cases of CIN Ⅲ and 30 cases of cervical squamous cell carcinomas. Results RT-PCR results showed that ACP was higher expressed in diseased cervical tissues than that in normal cervix. Immunohistochemical results indicated the expression of ACP was increasingly higher with the progress of the cervical lesions , as well as the result of Western blot. Simultaneously, it was demonstrated that ACP protein localized in cytoplasm. Conclusion The expression of ACP in the progress of the cervical lesions is increasing remarkably, and ACP may be considered as a auxiliary diagnosis index of CIN and cervical squamous cell carcinomas.%目的 了解IL-1F10-3基因rs3811058位点的单核苷酸多态性与强直性脊柱炎遗传易感性的关系.方法 采用病例对照研究设计,通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对基因型筛查并进行基因组测序验证,检测151例强直性脊柱炎患者和155例正常对照基因型,并分析基因型及等位基因频率在两组中的分布.结果 IL-1F10-3基因rs3811058位点的等位基因频率和基因型频率在病例组和对照组中的分布差异均无统计学意义.结论 安徽地区汉族人群强直性脊柱炎患者遗传易感性可能与IL-1F10-3基因rs3811058位点单核苷酸多态性无关.