胸腺素beta-4在肝母细胞瘤肝组织中的表达及其临床病理意义

摘要

Objective To investigate the expression of thymosin beta4 ( Tβ4 ) in the liver tissues of children with hepatoblastoma ( HB) , and further study the function of Tβ4 in HB metastasis in vitro. Methods Immunohisto-chemistry (IHC) was used to determine expression of Tβ4, E-cadherin andβ-catenin in liver tissues from 19 chil-dren with HB,and further to analyze its function in metastasis of HB cells. Results The positive rate of Tβ4 ( P<0.01 ) in HB liver tissues was significantly higher than it in the adjacent tissues. The positive rates of Tβ4 and nu-clearβ-catenin in HB with lymphnode metastasis were significantly higher than in HB without lymphnode metastasis ( P <0.01 ) . While expression of E-cadherin in HB with lymphnode metastasis was lower than in HB without lymphnode metastasis ( P<0.01 ) . Scratch-wound assay showed that HepG2 cells with Tβ4 knockdown had signifi-cantly lower metastatic ability (P<0.05). Conclusion Tβ4 plays an important role in HB metastasis, suggesting it is a potential target for HB metastasis.%目的研究肝母细胞瘤( HB)患儿肝组织中胸腺素beta-4(Tβ4)表达水平及其临床病理意义。方法利用免疫组化法检测Tβ4、E-cadherin和β-catenin在19例儿童HB及其瘤旁对照肝组织中的表达水平，进一步探索Tβ4对HB转移的具体功能。结果 HB患儿肿瘤肝组织中Tβ4表达阳性率显著高于其远端瘤旁组织( P<0.01)。 Tβ4和细胞核β-catenin在淋巴结转移患者表达阳性率明显高于无淋巴结转移患者(P<0.01)，而E-cadherin表达量却明显降低(P<0.01)。体外划痕实验结果表明Tβ4敲除后的HepG2细胞迁移能力显著小于非敲除细胞( P<0.05)。结论 Tβ4在儿童HB淋巴转移中发挥重要功能，是治疗HB转移恶化的潜在分子靶点。