风湿性心脏瓣膜病合并心房颤动患者中DNMT3 A与Kv1.5蛋白的表达变化及相关性研究

摘要

目的：探讨风湿性心脏瓣膜病合并心房颤动( AF)患者DNA甲基转移酶3A(DNMT3A)与超速延迟整流性钾通道蛋白(Kv1．5)表达水平变化及其相关性研究。方法将20例风湿性心脏瓣膜病接受瓣膜置换手术患者于体外循环前切取的右心耳组织作为研究对象,根据AF的定义将标本分为AF组(n=10)和窦性心律(SR)组(n=10),分别应用免疫组化、Western blot 法检测心房肌组织中 DNMT3A 和Kv1.5蛋白表达水平变化。结果免疫组化检测显示AF组与SR组相比,DNMT3A蛋白表达增加(P<0．05)；而Kv1.5蛋白表达减少(P<0．05)。同时,Western blot检测显示AF组与 SR 组相比,DNMT3A 蛋白表达增加(P <0．05),而Kv1.5蛋白表达减少(P<0．05)。相关性分析显示DNMT3A与Kv1．5之间存在一定负相关性(r =-0．64, P <0．01)。结论 DNMT3A与Kv1.5之间存在负相关性,提示DNMT3A可能参与调控Kv1．5通道蛋白的表达。%Objective To investigate the expression correlation between DNA methyltransferase 3 A ( DNMT3 A ) and rapid delayed rectifier potassium channel(Kv1. 5) in the patients with rheumatic heart disease and atrial fibril-lation ( AF) . Methods Tissues of auricula dextra were removed from 20 patients with rheumatic heart disease un-dergoing cardiac surgery for correction to valvular defect. According to the definition of AF ( n =10 ) ,20 patients were divided into groups of AF and of sinus rhythm(SR)(n=10). Expressions of DNMT3A and Kv1. 5 were de-tected by immunohistochemistry and Western blot. Results Compared with the group of SR, the expression of DN-MT3A protein was significantly enhanced (P<0. 05) whereas Kv1. 5 decreased(P<0. 05) in the results by immu-nohistochemistry. Meanwhile, the results from Western blot also showed increased DNMT3A(P <0. 05) and re-duced Kv1. 5(P<0. 05) in the group of AF. And negative correlation between the expression of DNMT3A and Kv1. 5 was shown with correlation analysis(r= -0. 64, P<0. 01). Conclusion The negative correlation exists in the expression of DNMT3 A and Kv1 . 5 , which indicates that DNMT3 A can regulate the expression of Kv1 . 5 .