吸烟对类风湿关节炎外周血芳香烃受体的影响

摘要

Objective To explore the possible reasons of the influence about smoking on rheumatoid arthritis (RA) through detecting the mRNA levels of aryl hydrocarbon receptor (AHR), its response gene cytochrome P4501Al (CYPlA1) and the repressor AHRR in peripheral blood mononuclear cells (PBMCs) of both patients and healthy controls.Methods Real-time PCR was used to assess the expression of peripheral blood AHR , AHRR, CYP1A1 from 58 patients with RA and 30 healthy subjects, then effects of smoking on the expression of AHR , AHRR and CYP1A1 were analyzed.Results The expressions of AHR, AHRR and CYP1A1 at mRNA level were significantly higher in patients who were smoking than those who were non -smokers(P <0.05).However, there were no statisti-cally significant differences between smoking healthy subjects and nonsmoking controls .Furthermore, we concluded that the expression of AHR was obviously increased in smoking RA patients than that in smoking healthy controls (P<0.05), and no significant differences were found between nonsmoking patients and nonsmoking healthy subjects . Conclusion Smoking is involved in the pathogenesis of RA and then becomes a risk factor which may influence the expression of AHR, AHRR, CYP1A1 in PBMCs from patients with RA.%目的：检测吸烟类风湿关节炎（RA）患者和健康者外周血单个核细胞（PBMCs）中芳香烃受体（AHR）、芳香烃受体抑制因子（AHRR）、细胞色素 P4501A1（CYP1A1）的表达水平，探讨吸烟影响 RA 发生、发展的可能原因。方法采用荧光定量 PCR 检测58例 RA 患者和30例健康者 PBMCs中 AHR、AHRR、CYP1A1 mRNA 的相对表达量，分析吸烟对3者表达的影响。结果RA 吸烟组 AHR、AHRR、CYP1A1 mRNA 的表达明显高于非吸烟组（P ＜0．05），而健康者吸烟组与非吸烟组 AHR、AHRR、CYP1A1 mRNA 的表达差异无统计学意义；RA 吸烟者 AHR mRNA 的表达明显高于健康吸烟者（P ＜0．05），而 RA 非吸烟者与健康非吸烟者之间差异无统计学意义。结论RA 中可能存在某种途径使 AHR 易被烟草化合物活化，进而致 AHR、AHRR、CYP1A1 mRNA 高表达，使香烟成为 RA 的危险因素。